Introduction: Learning methods are used to realize the applied learning strategies. Each learning method has its most prominent learning domain, such as cognitive (change of knowledge), affective (change of behavior), and psychomotor (change or improvement of skill). Midwifery is a profession that requires specific knowledge, attitudes, and skills that must be possessed and mastered. The process of achieving good competence of midwives starts with midwifery education. In achieving competence, midwifery students need theoretical and practical learning experiences in laboratory clinical skills. The Learning Pyramid developed by the National Training Laboratory shows that most students remember 90% of what they learn by teaching others. Objective: Determining the effect of the peer-assisted learning method application in laboratory skills for the physical examination of newborns learning. Method: This research used a Quasi-Experimental design with a pretest-posttest design. The sample was 30 students taken through the quota sampling technique, and the analysis used was the Wilcoxon Test. Result: The Peer-Assisted Learning method affected the learning outcome of skills in the physical examination of newborns with a significance level of P = 0.000 < 0.05. Conclusion: The Peer-Assisted Learning method improves the physical examination skills of newborns of DIII midwifery students.