Ximenia americana bark, leaves, and fruits which belong to the family Olacaceae traditionally used for the treatment of different human ailments. The study was conducted to evaluate the scientific basis for the use of the plant. Chemical constituents of the extract were also determined. The extract was active against different microbiological organisms were found. Phytochemical screening extract showed the presence of saponins, few alkaloids, tannins, flavonoids, terpenes, triterpenes sterols, and coumarins in all extracts and fractions. Anthrax-quinones, starch, general glycosides, and bitter principles were found to be present in the extract. The study supports the traditional use of this plant by herbalists as a remedy and curing different ailments.