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Purpose: The purpose of this research is to determine different factors explaining the intention of public in context of blood donation in the district of Jamshoro, Sindh. Methodology: The data were collected of 400 samples from four different tehsils of Jamshoro. The data was collected through well-structured questionnaire. The survey was conducted in 2019 and cluster sampling technique was used. The internal consistency of the questionnaire was examined, and Chi-Square test was applied for final analysis. Findings: The public's willingness to donate blood is limited, according to this study (χ2 (1) = 0.88, p=0.39). The media does not perform any significant role in awareness generating and educating the general public about the importance of donating blood (χ2 (1) = 24.35, p=0.001). Women make up a small percentage of blood donors in society (χ2 (1) = 0.05, p=0.82). This research also compares blood donors and non-donors based on gender and age. The contribution of blood donors in younger age was higher (χ2 (3) = 19.31, p=0.01) in males (χ2 (1) = 27.98, p=0.001). Conclusion: The awareness of blood donation was higher in males, higher education, and young age peoples (18-28) years. Furthermore, the awareness about blood donation should be made known through electronic and print media along with the educational programs at educational institutions. Blood bank lab assistants should be given training to keep up to date with the latest information on blood donation, samples, and screening tests.