From last few decades there is an upsurge in the use of herbal medicines all over the world and these herbal drugs should be studied scientifically to develop their monographs to assure their quality. The present research work was an attempt to establish parameters for identification of Plumeria obtusa L. (Fam. Apocynaceae) according to the guidelines of WHO, by studying its morphological and organoleptic characters, detailed microscopic evaluation, histochemical studies, fluorescence analysis, loss on drying, extractive values, swelling index, foaming index and preliminary phytochemical screening of the leaves and flowers. Although conventional, but it is simple, easy and quick way for identification and standardization of herbal substances affordable even in developing countries. This is the first report on pharmacognostic and physicochemical studies on P. obtusa which can be helpful in establishing pharmacopoeial monograph of this plant.