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<p class="Abstract">Hamelia patens is used in folk medicine in the treatment of nervous shock. The present study deals to evaluate the antidepressant-like effects of chloroform and methanol extracts of H. patens on the performance of male mice and GC-MS profiling of bioactive extract. Mice were given extracts orally in acute doses of 100 and 200 mg/kg daily for 7 days and then subjected to forced swim test, tail suspension test and open field test. Imipramine (10 mg/kg/day, p.o.) and  fluxetamine (10 mg/kg/day, p.o.) were used as the standard in forced swim test and tail suspension test  respectively. GC-MS profiling of chloroform extract was performed to find out the chemical constituents in bioactive fraction. After one-week treatment, the chloroform extract (100 and 200 mg/kg/day, p.o.) significantly reduced immobility time in forced swim test and tail suspension test (p&lt;0.05). All extracts did not show any significant change in the locomotor activity in open field test. These data indicate that the extract of H. patens possesses antidepressant-like properties in mice without any significant effect on locomotor activity.</p><p><strong>Video Clip of Methodology</strong>:</p><p>4 min 06 sec:   <a href="https://www.youtube.com/v/DDJzDqiwCKU">Full Screen</a>   <a href="https://www.youtube.com/watch?v=DDJzDqiwCKU">Alternate</a></p>

Amaç: Bu çalışmada, Ixora coccinea ekstrelerinin sıçanlardaki antidepresan etkisi araştırılmış ve GC-MS tekniği kullanılarak aktif ekstrenin fitokimyasal analizi yapılmıştır. Gereç ve Yöntemler: Ekstrelerin oral yolla uygulanmasının ardından, antidepresan etkinin değerlendirilmesi için kuyruktan asma testi (KAT), zorunlu yüzme testi (ZYT) ve açık alan testi (AAT) kullanılmıştır. Biyoaktif ekstredeki fitokimyasal bileşenleri aydınlatmak amacıyla I. coccinea metanollü ekstresi üzerinde GC-MS analizi yapılmıştır. Bulgular: I. coccinea metanollü ekstresi 100 ve 200 mg/kg dozda, oral yolla uygulandığında, KAT ve ZYT testlerinde toplam hareketsizlik süresini anlamlı bir şekilde azaltmıştır (p<0.01). I. coccinea ekstreleri AAT'de lokomotor aktivite üzerinde anlamlı derecede bir etki göstermemiştir. Sonuç: I. coccinea metanollü ekstresi sıçanlarda, AAT'de anlamlı derecede lokomotor aktiviteye neden olmadan antidepresan-benzeri etkiye sahiptir. Anahtar kelimeler: Antidepresan aktivite, zorunlu yüzme testi, GC-MS, Ixora coccinea, açık alan testi, kuyruktan asma testi Objectives: The present study aims to assess the antidepressant activity of Ixora coccinea extracts in mice and phytochemical analysis of the active extract by GC-MS. Materials and Methods: After oral administration of extracts, tail suspension test (TST), force swim tests (FST), and open field tests (OFT) were performed to assess the antidepressant activity. GC-MS analysis of methanol extract of I. coccinea was performed to ascertain the chemical constituents in the bioactive extract. Results: The methanol extract of I. coccinea at dose of 100 and 200 mg/kg body weight, p.o. significantly reduced the total duration of immobility in the TST as well as FST (p<0.01). I. coccinea extracts showed no significant changes in locomotor activity in OFT. Conclusion: The methanol extract of I. coccinea possesses antidepressant-like properties in mice with no significant effect on locomotor activity in OFT.

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