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The high nitrogen values for the washings are due to non-antibody protein of the serum. The progressive reduction in the values indicates the removal of this protein by successive dilution. The possibility that non-antibody protein was non-specifically taken up in the lattice of agglutinated ghosts cannot be excluded. Therefore a portion of the nitrogen in the resuspended antibody may not repre-sent antibody nitrogen.Summary. Study of a human cold hemagglutinin revealed:1. The cold hemagglutinin had the electrophoretic mobility of gamma globulin. 2. A cold hemagglutinin titer of 1/2560 at 4OC was equivalent to 1.473 mg per ml of antibody nitrogen.