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Tuberculosis (TB) is one of the major concerns of health policy and rapid detection of M. tuberculosis and detection of rifampicin (RIF) resistance in infected patients are essential for disease management. Multi-drug resistance (MDR) TB is dened as tuberculosis (TB) disease caused by a strain of Mycobacterium tuberculosis (MTB) that was resistant to at least isoniazid and rifampicin (RIF). The aim of this study was to evaluate the importance of GeneXpert MTB/RIF for detection of Mycobacterium tuberculosis (MTB) with detection of rifampicin (RIF) resistance. In this study 967suspected cases of pulmonary and extra-pulmonary TB were included. Their sputum and other (Pus, CSF, tissue, etc.,) samples were collected and subjected to smear microscopy test followed by GeneXpert MTB/RIF assay. Of total 967 samples, 470 (48.60%) were positive for Mycobacteria by GeneXpert MTB/RIF and 118 (12.20%) samples were positive by ZN smear. Among 967 positive cases, 818 (84.59%) were pulmonary, 149 (15.41%) were extra-pulmonary (EP). Two ZN smear positive cases were negative by GeneXpertMTB/RIF. Of the 470 GeneXpert positive samples for MTB, 29(6.17%) cases showed RIF resistance and diagnosed as MDR-TB. GeneXpert MTB/RIF assay is efcient and reliable technique for the rapid diagnostic of TB. The GeneXpert MTB/RIF assay is a simple, high sensitivity and specicity for RIF resistance detection implies for diagnosis of MTB and RIF resistance in MDR cases as well as suspected smear negative and extra-pulmonary cases.