The objective of the present study was to isolate rhizospheric bacteria from samples from the Santa Cruz Mine, department of Bolívar, in order to evaluate in vitro resistance activity at different concentrations of mercury. The samples were collected randomly from different sites near the Santa Cruz gold mine, from which they isolated rhizospheric bacteria. The resistance capacity of bacteria at different concentrations of mercury in the form of mercury chloride (HgCl 2 ) at concentrations of 50 ppm, 100 ppm, 150 ppm, 200 ppm and 250 ppm was evaluated in vitro; also the bacteria resistant to this metal were used to evaluate the ability to promote growth in plants. The results showed that the bacterium identified as Pseudomonas luteola, resisted in vitro at 200 ppm of mercury chloride (HgCl2), and qualitatively had the ability to produce siderophores and biologically fix nitrogen. P luteola was isolated from the rhizosphere near the Santa Cruz mine in the department of Bolívar, Colombia, with soils with high concentrations of mercury and extremely acidic soil reaction. Keywords:Bacteria; rhizosphere; mercury; resistance. ResumenEl objetivo del presente estudio fue aislar bacterias rizosféricas de muestras provenientes de la Mina de Santa Cruz, departamento de Bolívar, con el propósito de evaluar in vitro la actividad de resistencia a diferentes concentraciones de mercurio. Las muestras fueron recolectadas aleatoriamente de diferentes sitios de cerca de la mina de oro Santa Cruz, a partir de la cuales de aislaron bacterias rizosférico. Se evaluó in vitro la capacidad de resistencias de las bacterias a diferentes concentraciones de mercurio en forma de cloruro de mercurio (HgCl 2 ) a concentraciones de 50 ppm, 100 ppm, 150 ppm, 200 ppm y 250 ppm; así mismo las bacterias resistentes a este metal se utilizaron para evaluar la capacidad de promoción de crecimiento en las plantas. Los resultados mostraron que la bacteria identificada como Pseudomonas luteola, resistió in vitro a 200 ppm de cloruro de mercurio (HgCl 2 ), y cualitativamente tuvo la capacidad de producir sideróforos y fijar biológicamente nitrógeno. P luteola fue aislada de la rizósfera cerca de la mina de Santa Cruz en el departamento de Bolívar, Colombia, con suelos con altas concentraciones de mercurio y con reacción del suelo extremadamente acida. Palabras Clave:Bacteria; rizósfera; mercurio; resistencia.Presencia de bacterias rizosféricas resistentes a mercurio en suelos del sur de Bolívar, Colombia
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