BACKGROUND Chronic Suppurative Otitis Media (CSOM) is a common infectious chronic ear disease in India. The present study of Department of ENT OPD of ESIC-MC-PGIMSR, Bangalore, was aimed to identify aerobic bacterial isolates associated with CSOM and their antibiogram in patients. MATERIALS AND METHODSProspectively studied one hundred and thirteen patients with CSOM with ear swabs taken and cultured for bacteria. The standard method of isolation and identification was followed. Antimicrobial susceptibility of the bacterial isolates was performed by KirbyBauer's disc diffusion method. RESULTSPseudomonas aeruginosa 28 (49.1%) was the most predominant organism followed by Staphylococcus aureus 16 (28%). Fungi accounted for 3 (2.6%) of the isolates. The antimicrobial profile of the major isolates showed 75% of Pseudomonas aeruginosa and 62.5% of Staphylococcus aureus sensitive to ciprofloxacin. CONCLUSIONPseudomonas aeruginosa is the most common isolate followed by Staphylococcus aureus. The study of microbial pattern and their antibiotic sensitivity determines the prevalent bacterial organisms causing CSOM in local area and to start empirical and more targeted treatment of otitis media and its complications for successful outcome, thus to prevent the emergence of resistant strains.