A total of twenty-six local chickens were representing shank feathering and non-feathering shank were used to sequence five QTLs, which associated with shank feather trait in chicken. The five location sequence results were shown polymorphism between the shank feathering and nonfeathering shank. All the candidate markers were differed between the shank feather and nonfeathering shank. The big distance was in (ADL221), and the less distance was in marker MCW315.