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Development of a method of preserving suspension cultures of plant cells has been undertaken. When grown in a conventional medium containing 20 g/1 of sucrose, Daucus carota cells survived freezing and a short period of storage at −40 °C or −196 °C in the presence of a suitable protective agent, whereas Ipomoea sp. cells did not. Ipomoea cells survived freezing if they were adapted to growth in a medium containing 65 g/1 of sucrose.

The vapor pressure of hypostoichiometric urania was measured as a function of temperature for a series of compositions. The data were obtained by a n entrainment technique in which weight loss of a specimen vaporizing at high temperatures in a stream of carrier gas is followed continuously with a recording thermobalance. Use of suitable atmospheres produced constant vaporizing compositions which vaporized pseudocongruently. Equations obtained for the vapor pressure are :59,100 f . 1000UOl 88 In Pdv (atm) = 15.2 =k 0.4 -____-___