A 28-year-old nulligravida woman with regular menstruation previously presented with 2 months of amenorrhea, right lower abdominal pain, and vaginal bleeding for 1 day. On clinical examination, the patient was hemodynamically stable. Abdominal examination revealed right iliac fossa tenderness. Speculum examination revealed minimal vaginal bleeding. On vaginal examination, a normal-sized uterus with an adnexal mass in the right fornix was observed. Cervical motion tenderness was present. A urine pregnancy test was positive. The quantitative beta human chorionic gonadotropin was 15 146 IU/mL. A transvaginal ultrasound scan was performed, which showed no evidence of an intrauterine pregnancy with an empty uterus filled with fluid. There was a right tubal unruptured ectopic pregnancy with a gestational sac 34 mm in size; a yolk sac was noted. The crown rump length was 24 mm with a fetal heart rate of 164 beats/min. The estimated gestational age was 8 weeks 4 days based on the ultrasound findings. Laparoscopic examination was performed, which revealed a 5 × 3 cm right fallopian tubal unruptured pregnancy in the ampulla with a 1 × 1 cm right tubal mass in the isthmus (Fig. 1). A right salpingectomy was performed followed by a peritoneal lavage (Fig. 2). The 1 × 1 cm mass was whitish, firm, and incompressible in nature (Fig. 3). The mass was extruded in the pelvis during the salpingectomy. Both the ovaries and the left tube were normal. The right fallopian tube and the mass were sent for histopathological examination, which confirmed the presence of a gestational sac and a 1 × 1 cm leiomyoma in the tube along with the ectopic pregnancy. The coexistence of a tubal leiomyoma and a tubal ectopic pregnancy is extremely rare [1]. Tubal leiomyomas may predispose to tubal implantation of a conceptus because of The authors declare that they have no conflict of interest.
Background: Fusarium oxysporum f. sp. ciceris causing chickpea wilt, a significant soil-borne disease that causes severe crop losses. Trichoderma spp. is a diverse fungal bio-control agent with the ability to limit plant disease growth through a variety of ways. The goal of this work is to assess the efficiency of indigenous Trichoderma spp. isolated from chickpea rhizosphere from different North Eastern districts of Karnataka against the wilt pathogen in vitro, as well as to investigate the cultural and morphological aspects of isolates.
Methods: Dual culture approach was used to investigate the antagonistic potential of Trichoderma isolates and inverted plate technique was used to examine the synthesis of volatile compounds from Trichoderma spp. The isolates were cultured on potato dextrose agar medium for cultural characterization. Under a microscope, morphological characters were observed.
Result: Twenty Trichoderma isolates were reported to be effective against F. oxysporum f. sp. ciceris, preventing mycelial growth by 48.52 to 91.85%. All the isolates produced significant amount of volatile compounds that inhibited F. oxysporum f. sp. ciceris growth by 2.59 to 72.22%. At 72 hours after incubation, the isolates had different radial mycelium growth and formed fluffy and elevated colony growth, with colony margins ranging from smooth to wavy.
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