ABSTRAKSalah satu upaya untuk mencegah pemasukan atau penyebaran organisme pengganggu tanaman adalah melalui perlakuan fisik terhadap komoditas pertanian sebagai perlakuan pra dan pasca panen. Penelitian bertujuan untuk mendapatkan metode eradikasi cendawan pada biji kedelai sebagai metode perlakuan karantina yang efektif. Sebagai cendawan model dipilih Cercospora sp., Sclerotium rolfsii, Colletotrichum gloeosporiodes, dan Pestalotia sp. Pengujian dilakukan dengan udara panas pada 45, 50, 55, 60, 65, 70 °C dan kontrol selama 5 jam terhadap benih, biji, dan bungkil kedelai dan isolatisolat cendawan model. Perlakuan udara panas secara umum menyebabkan penghambatan secara nyata terhadap perkecambahan dan pertumbuhan benih kedelai. Walaupun demikian, perlakuan suhu 45, 50 dan 55 °C menyebabkan jumlah biji yang terinfestasi cendawan lebih rendah, bahkan suhu 65 dan 70 °C menyebabkan cendawan tidak tumbuh pada permukaan biji.Kata kunci: eradikasi, perlakuan karantina, udara panas ABSTRACT Introduction and dissemination of plant disease agents can be avoided through physical treatment on agricultural commodities as pre and post harvest treatment. Research was conducted to find effective quarantine treatment to eradicate fungi infestation on soybean seed. Four fungi species were chosen for this study, i.e. Cercospora sp., Sclerotium rolfsii, Colletotrichum gloeosporiodes, and Pestalotia sp. The evaluation was conducted for seed, seedling, bean oilcake, and fungi culture using hot air treatment on 45, 50, 55, 60, 65, 70 °C and control for 5 h. In general, heat treatment caused suppression significantly on seed germination and seedling growth. However, heat treatment on 45, 50 and 55 °C resulted on lower infestation of fungi on seed, and no infestation was observed on 65 and 70 °C

The importation of corn seeds may inadvertently introduce seed-borne fungi like Aspergillus niger. One potential method to control this pathogen was fumigation with sulfuryl fluoride. The objectives of this applied research were to obtain effective concentration and exposure time of sulfuryl flouride as a fumigant to control seedborne fungi and to its physiological impact on corn seeds. Aspergillus niger was isolated from corn seed samples, grew it in potato dextrose agar, and used it for in vitro studies. Parameters  observed were isolates’ growth inhibition. In vivo studies were conducted using corn seed samples infected by Aspergillus niger. Sulfuryl fluoride was applied at the concentration of 30, 40, 50, and 60 g/m3 and 24, 48, 72, and 96 hour of exposure time. Results showed that sulfuryl fluoride concentration of 40 g/m3 for 48 hours at 26-32°C is the only effective concentration against A. niger in vitro but all of the treatment did not significantly affect A. niger in vivo. Concentration of 30, 40, 50, 60 g/m3 for 24 hours does not affect the quality of the seed.