Objective: This study aimed to synthesize a new iron nanoparticles derived from beta vulgaris extract and test its bioactivity against certain microbes. Materials and Methods: preparation of extract and the nanoparticles were done using certain procedures, while the Identification of Entero cloacae VITEK-2 ID-GN and AST-N 020 cards correspondingly applied for detection and susceptibility tests direct as of positive BacT/ALERT FA and FN blood cultures. Antibiotic susceptibility testing and anti bacterial as well as anti inflammatory activity, inhibition of protein denaturation and anti oxidant efficiency were achieved using AFM, UV-Visible and other instruments. Results: the presence of antioxidants, antibacterial and anti-inflammatory materials in nanoparticles were much more than in the extract. Conclusions: iron nanoparticles show much more activity against bacteria, oxidant and inflammatory agents.