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In vitro shoots from mature tree explants often show poor root initiation response. Garcinia species are known for vegetative propagation through root suckers in natural environment. To establish a protocol for clonal propagation, apical and intercalary buds of the root suckers obtained from a mature elite tree of Garcinia indica were used as explant source. Multiple shoots were initiated in woody plant medium fortified with combinations of 6-benzylaminopurine and 1-phenyl-3-(1,2,3-Thiadiazol-5-YL)-urea. A pulse treatment of indole-3-butyric acid ranging from 4.9 to 19.6 mM for 30 s and 1 min was tried for root induction. The resulting in vitro plants were successfully hardened. The initiated shoots were also used for in vitro synthesis of hydroxycitric acid in shake flask cultures. The effect of basal medium, incubation period and plant growth regulators on production of hydroxycitric acid in vitro was studied.