The third stage larvae (L3) of <i>Anisakis typica</i> were detected in 2 species of threadfin bream, <i>Nemipterus hexodon</i> and <i>N. japonicus</i>, from the Gulf of Thailand, and were morphologically and molecularly characterized. Total 100 threadfin breams, 50 <i>Nemipterus hexodon</i> and 50 <i>N. japonicus</i>, were examined with naked eyes after the opening of abdominal cavity with scissors. Almost all infected larvae remained alive and active even the fish were transported for 1-2 days. Anisakid larvae were exclusively distributed in the body cavity and rarely in the liver. The prevalence of <i>A. typica</i> L3 were 68.0% and 60.0% in <i>N. hexodon</i> and <i>N. japonicus</i> and their infection intensities were 3.5 and 4.2 per fish infected each. Morphological and morphometric analysis were performed by viewing specimens under both a light microscope and a scanning electron microscope. Interestingly, the protruded mucron of <i>Anisakis typica</i> under SEM showed a distinct cylindrical shape that differed from the cone shape of <i>A. simplex</i>. The protruded mucron could be used to identify <i>A. typica</i> L3 larvae in the future. A comparison of the ITS1-5.8S-ITS2 rDNA nucleotide sequences of these species revealed high blast scores with <i>A. typica</i>. Conclusively, it was confirmed that <i>A. typica</i> L3 are prevalent in threadfin breams from the Gulf of Thailand, and their morphological and molecular characters are something different from those of other anisakid larvae, including A. simplex and <i>A. pegreffii</i>.