Surimi is considered a healthy food. Hypocholesterolemic and antioxidant properties of soluble fiber and microalgae may reduce cardiovascular disease risk, making them potential functional ingredients. This study aims to determine whether glucomannan-enriched or glucomannan plus spirulina-enriched squidsurimi improve plasma lipid/lipoprotein levels and arylesterase (AE) activity in Zucker Fa/Fa rats. Six groups of eight growing rats were given modified AIN-93M diets for 7 wk. Experimental diets included 30% squid-surimi control diet (C), 30% glucomannan-enriched squid-surimi diet (G), 30% glucomannan plus spirulina-enriched squid-surimi diet (GS), and the same diets enriched with hypercholesterolemic agent (2% cholesterol and 0.4% cholic acid; HC, HG, and HGS diets). G and GS diets induced significantly lower (p < 0.001) body weight gain and growth rate than the C diet and partially normalized the lipoprotein lipid contribution to plasma lipids. HC rats presented marked hyperlipemia, elevated cholesterol/triglyceride ratio in all lipoprotein fractions, and a high percentage of cholesterol transported by VLDL and IDL þ LDL fractions. HG and HGS diets noticeably normalized lipemia and the lipid composition of the various lipoprotein fractions versus HC. In conclusion, glucomannan added to surimi-diets decreased bodyweight gain. HG or HGS exhibited strong lipid normalizing properties versus HC. Spirulina did not add to the effects of glucomannan.