The focus of the present work is on the design, synthesis, characterization, DNA-interaction, photo-cleavage, radical scavenging, in-vitro cytotoxicity, antimicrobial, docking and kinetic studies of Cu (II), Cd (II), Ce (IV) and Zr (IV) metal complexes of an imine derivative, 3 - (1 - (6 - methoxybenzo [d] thiazol - 2 - ylimino) ethyl) - 6 - methyl - 3H - pyran - 2, 4 - dione. The investigation of metal ligand interactions for the determination of composition of metal complexes, corresponding kinetic studies and antioxidant activity in solution was carried out by spectrophotometric methods. The synthesized metal complexes were characterized by EDX analysis, Mass, IR, (1)H-NMR, (13)C-NMR and UV-Visible spectra. DNA binding studies of metal complexes with Calf thymus (CT) DNA were carried out at room temperature by employing UV-Vis electron absorption, fluorescence emission and viscosity measurement techniques. The results revealed that these complexes interact with DNA through intercalation. The results of in vitro antibacterial studies showed the enhanced activity of chelating agent in metal chelated form and thus inferring scope for further development of new therapeutic drugs. Cell viability experiments indicated that all complexes showed significant dose dependent cytotoxicity in selected cell lines. The molecular modeling and docking studies were carried out with energy minimized structures of metal complexes to identify the receptor to metal interactions.

Diabetes mellitus is one of the most common metabolic disorders of multiple etiology. The multisystem effects of diabetes such as retinopathy, nephropathy, neuropathy and cardiovascular diseases have an important impinging on the working age individuals in our country. Diabetic Retinopathy (DR) is one of the leading causes of blindness in the world that increases the chance of losing vision to about 25 times higher compare to normal individuals. Diabetic Nephropathy (DN) is the major life-threatening complication which develops in approximately 20% to 40% of type 1 and less than 20% of type 2 diabetic patients. DN is the leading known cause of End stage renal disease (ESRD). AIM AND OBJECTIVE: A systematic cross-sectional study was conducted in Sarojini Devi eye hospital to assess the correlation between Diabetic Retinopathy and Diabetic Nephropathy in Type II Diabetes Mellitus patients. METHODS AND MATERIALS: A study was conducted on 108 Type II DM patients presenting to Sarojini Devi Eye hospital, Hyderabad and were assessed for Retinopathy and Nephropathy and Correlation between Retinopathy and Nephropathy was studied from December 2011 to December 2014. RESULTS: Out of 54 Diabetic Retinopathy patients, 28 (51.8%) patients had DN, 26 patients (48.2%) had no evidence of DN. Out of 54 Diabetic Nephropathy patients, 36(66.6%) had DR; 18(33.4%) had No evidence of DR. CONCLUSION: The results of our study suggest that Diabetic Nephropathy has a positive association with the presence of Diabetic Retinopathy in persons with Type II DM.

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The compound N-(2-hydroxybenzylidene)-1-ethyl-1, 4-dihydro-7-methyl-4-oxo-1, 8 naphthyridine-3-carbohydrazide (LH) and its Cu (II), Co (II) and Zn (II) complexes were synthesized and characterized. The absorption spectral titrations and competitive DNA binding studies depicted those complexes of title compound bind to CT-DNA through intercalation. Interestingly [Cu (II)-(L2)] showed relatively high binding constant value (6.61 x 10(5) M(-1)) compared to [Co (II)-(L2)] (4.378× 10(5) M(-1)) and [Zn (II)-(L2)] (3.1x10(5) M(-1)). Ligand and its complexes were also examined for DNA nuclease activity against pBR-322 plasmid DNA, which showed that [Cu (II)-(L2)] had the best hydrolytic cleavage property displaying prominent double-strand DNA cleavage. In addition, antioxidant activities of the ligand and its metal complexes investigated through scavenging effects for DPPH radical in- vitro, indicated their potentiality as good antioxidants. The in vitro anti-bacterial study inferred the better anti-bacterial activity of [Cu (II)-(L2)] and this was also correlated theoretically by employing docking studies wherein [Cu (II)-(L2)] displayed good Gold score and Chem score. Finally the in vitro anti- proliferative activity of studied compounds was tested against HeLa and MCF-7 cell lines. Interestingly [Cu (II)-(L2)] displayed lower IC50 value and lower percentage of viability in both HeLa and MCF-7 cell lines.