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Background:New biosensor techniques allowing detection of low concentrated substances show a great variety nowadays. The construction of a system with modified quartz as a part of a Quartz Crystal Microbalance (QCM) techniques helps the detection and confirmation of low toxin concentrations in a sample. Objective:The study aims to allow the application of methods for preparation and modification of the gold surface of piezoelectric crystal for detection of aflatoxin B1(AFB1) in the concentration range 0.2 -2.0 µg/L by QCM technique (quartz -crystal microbalance). Methods:The procedure for the preparation of quartz crystal sensors for experimental purposes was performed. The quartz surface was activated and covered with self-assembled monolayer to immobilize antibody (rabbit anti-aflatoxin B1) for the detection of antigen -antibody reaction. Results:The G" corresponds to viscous properties of the material, during applied deformation of the material in the presence of different concentrations, which revealed in the sensitivity of the used resonator. Conclusion:Detection of toxic pollutants may be achieved via QCM methods, ultrasound resonator and piezoelectric quartz techniques for measurement. These techniques allow detection of significantly low concentrations of toxic pollutants, in particular, AFB1, compared to analysis with direct and indirect ELISA immunoassays.

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This paper continues series of research on application of the novel approach of InterCriteria Analysis (ICrA) to medical data. It describes a new method of analyzing the treatment results of patients with Behterev's disease in order to aid the decision making process for treatment course-InterCriteria Analysis. The ICrA analysis is applied on the results of medicine, physiotherapeutic treatment and kinesitherapeutical program characteristics. The main goal is an improvement of general quality of patients' life through practices of specific methodology of kinesitherapy and ergotherapy. The object of empirical study is health status of patients suffering Rheumatoid spondylitis in relation to their current life conditions. Here are analyzed the data from observation about 25 patients (14 women and 11 men, aged 25 to 67). The results confirm previously performed research, showing that the muscles in the human body are closely connected and the improving of one movement will lead to improving another. The obtained results also prove applicability of ICrA to the researched problem, give grounds for extending its application and potential of further in-depth study.