We investigated the ability of western equine encephalitis virus envelope glycoproteins (WEEV GP) to pseudotype lentiviral vectors. The titers of WEEV GP-pseudotyped human immunodeficiency virus type 1 (HIV) ranged as high as 8.0 ؋ 10 4 IU/ml on permissive cells. Sera from WEEV-infected mice specifically neutralized these pseudotypes; cell transduction was also sensitive to changes in pH. The host range of the pseudotyped particles in vitro was somewhat limited, which is atypical for most alphaviruses. HIV vectors pseudotyped by WEEV GP may be a useful tool for characterizing WEEV cell binding and entry and screening for small-molecule inhibitors.

of the liver of the rats fed mannitol and cacao-butter ranged from 0.62 to 1.80% with an average value of 1.23%, as compared with the average glycogen content of the livers of control rats fed cacaobutter alone of 0.14%.It is impossible to compare the results of this series with our own experiments since the treatment of the animals was quite different in the 2 series. Under the experimental conditions used by us, it is evident that mannitol did not serve as a ready available source of glycogen. A similar lack of ready utilization of mannitol is shown by the reported failure of mannitol to relieve insulin shock in white rats." 7082 C