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The Diagnosis of hydatidosis is still an unsolved issue due to difficulties in obtaining of patient's hydatid cyst appropriate for antigen extraction. This study evaluated the suitability of HC protoscolices somatic antigens (HCPsS-Ag) fractions from animal origin to substitute that extracted from HC of patients in diagnosis of hydatidosis using enzyme-linked immunoelectro-transfer blot and Enzyme-linked immunosorbent assay (ELISA). Eight fractions in HC-G6 from patients react specifically versus HC-G6 infected patient's sera. Five of them (28, 32, 38, 59 and 89 Kilo Dalton (KDa) and two of them (28 KDa and 45 KDa) reacted versus HC-G1 and HC-G4 infected sheep and equine sera, respectively. Six fractions in HCPsS-Ag-G1 of sheep react versus HC-G1 sheep infected sera, four (28, 32, 52 and 58 KDa) and two of them reacted versus HC-G6 and HC-G4 infected patient and equine sera, respectively. Two fractions only in HCPsS-Ag-G4 of equine reacted versus infected human and sheep sera. This fraction displayed the same degree of ELISA value versus different infected sera with a significantly perfect classification for kappa agreement and non-statistically significant difference (p C 0.05) for ELISA Optical density values of the positive samples without cross-reaction versus other parasites antibodies in sera. HCPsS-Ag from HC genotypes that developed in humans and animals as HC-G6 and HC-G1 can substitute each other for diagnosis of infection than antigens extracted from non-zoonotic HC-G4. The fraction at 28 KDa is the only fraction that can be extracted from any animals HC and used in diagnosis of zoonotic hydatidosis.

Cymothoids are obligate parasites of marine, brackish and freshwater fishes. They were considered to cause significant economic losses to fisheries through mortalities, stunting growth or damaging tissues of the infested fish. In the last 4 years, lake Qarun suffered from isopod infestation causing fish loss and marketing problems. In the present study out of 375 fish samples belonged to Mugil capito (125), Liza carinata (125) and Tilapia zilli (125) were collected from lake Qarun , El Fayoum governorate and seasonally examined for isopod infestation from the beginning of November 2015 till the end of October 2016. The study revealed the isolation of two species of isopods ; Nerocila orbignyi and Levonica redmani with total infestation rate of 32.53% (21.07% and 11.47% for Nerocila orbignyi and Levonica redmani respectively) with the highest rate recorded during summer. Histopathological study was conducted for estimating the impacts of L. redmani infestation on the affected tissues of M. capito and revealed complete sloughing of the gill lamellae with eosinophilic granular cells infiltration. The study recommended the need to develop a scientific strategy to control the parasitic isopods and prevent its spread among Egyptian water bodies.