ant, J, V, 1996, Embryogenic cell suspensions from tiie male flower of Musa AAA cv. Grand nain. -Physiol, Plant, 97; 285-290, There are very few reports on the establishment of iong-term embrj'ogenic cell cuitures of banana, especially of triploid cultivars of commercial interest, Embryogenic cell suspensions were prepared using the cuitivar Grand nain, the most widely grown dessert banana in the world. After culture for 5 or 6 months of immattire male flowerbuds adjacent to the floral apex, yeilow, compact calluses and white, friable embrj'Ogenic tissues were induced. Suspension cultures were initiated ffom embryogenic tissues placed in liquid medium. The packed cell volume (PCV) of the suspensions increased 2-to 5-fold with each monthly culture cycle. Plating of tiie embryogenic suspensions resulted in approximately 370X10' embrj'os per ml of PCV, Depending on the size of embryos, 3 to 20% germination was observed, A histological survey of cell suspensions and embryo development was carried out. Cellular aggregates with cells displaying typical embryogenic features were formed. Most of the somatic embiyos were probably of unicellular origin. ), R. Domergue, S. Monmarson, J. Schwendiman and C Teisson. CIRAD-FLHOR. Laboratoire BIOTROP. B.P. 5035. Av. du val de Montferrand,

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