Back ground: Management program about infection control is important to help head nurses gain knowledge to perform their roles, and for nurses to comply with standard infection control measures. Objective: Evaluate efficacy of implementing management program about infection control practices for nursing staff. Setting: All departments in Tanta University Emergency Hospital. Subjects: All head nurses (N= 31) and nurses (N =175) working in all departments of emergency hospital. Tools: (1) Infection Control Knowledge Questionnaire sheet, (2) Head Nurses' Performance Role on Infection Control Assessment Scale and, (3) Observational checklist, related to Infection Control practices. Results: Head nurses 58.1% and nurses 80.0% had poor level of total knowledge about infection control pre-program , while majority (90.3%-86%) were at high level post program. Head nurses 61.3% had poor level of total specific knowledge pre-program, changed to be 90.3% and 87.1% had good level of knowledge post program. Pre-program majority (90.3%) of head nurses had mild level of performance of educational , consultation, research and support team in surveillance roles decreased to (3.2%) post program. Nurses range (15.4%-22.9%) showed compliance level to infection control practices pre-program, improved to range (75.4%-84.0%) post program with highly statistically significant differences (p= < 0.001). Conclusion: At Tanta University Emergency Hospital nursing staff basic knowledge, head nurses specific knowledge and role performance and nurses' compliance were low and statistically improved after implementation of needed management program. Recommendation: conduct regular training programs for nursing staff and encourage culture of compliance to infection control practices.