Purpose: The current study was conducted to elaborate the clinical, osteocalcin biomarker radiographic effect of biomodulaion effect of low intensity laser as an adjunct to mechanical debridement in the treatment of patient suffering of chronic periodontitis. Material and methods: This current study was conducted on twelve (12) healthy patients that were alienated randomly into two groups: Group A (non lased, control group) twelve (12) patients side with one stage mechanical debridement without using of the low intensity laser as an adjunctive treatment; Group B (Test group, lased) twelve (12) patients side with three stage treatment high level laser decontamination, mechanical debridement with the application of biomodulation six sessions of low intensity laser. Results: lased group showed a significant increase in the clinical parameter, osteocalcin biomarker level after three and six months in comparison with control group after three and six months follow up. Conclusion: The using of laser decontamination, low intensity laser with conventional mechanical debridement showed an improvement in the treatment outcome of chronic periodontitis.