Inhalt Der Embryo derived Platelet activating Fakcor (EDPAF) und der Early Pregnancy Factor (EPF) sind derzeit die frühesten nachweisbaren trächtigkeitsassoziierten Faktoren beim Rind. Rinderembryonen, die innerhalb einer 24 stündigenKulturzeit EDPAF (P < 0,02) produzierten, konnten erjolgreich übertragen werden. Der Transfer von Embryonen, die keinen EDPAF sezernierten, führte dagegen nicht zu einer Trächtigkeit. Platelet activating Factor (PAF) embryonalenund maternalen Ursprungs wurde im Serum tragender Rezipienten (n = 5) am d 1 (P < 0,05), d 5 (P < 0,02) und d 12 (P< 0,05) indirect mit Hilfe eines Bioassay nachgewiesen. Demgegenüber zeigten drei Rezipienten, die nicht tragend waren, keine PAF‐Aktivität im Serum. In Seren derselben Tiere wurde die EPF‐Aktivität mit einem indirekten ELISA bestimmt Signifikante Differenzen zwischen den tragenden und nicht tragenden Rezipienten wurden am Tag 1 und Tag 9 nach dem Transfer ermittelt.
Contents: Investigations of embryo‐derived platelt activation factor and early pregnancy factor in bovine after embryo transfer
At the earliest developmental stage embryos derived platelet activating factor (EDPAF) and the early pregnancy factor (EPF) can be used to detect pregnancy. Bovine embryos producing higher levels of EDPAF within 24 hours of culture (P < 0,02) were transferred successfully. Transfer of embryos that did not secret EDPAF never resulted in a pregnancy. PAF of maternal or embryonic origin was detected in the serum of pregnant recipients (n = 5) by means of an indirect bioassay at day 1 (P < 0,05) day 5 (P< 0,05) and day 12 (P < 0,05). In three recipients which did not become pregnant PAF was not activated in serum. Within the same animals EPF activity was determined by means of an indirect ELISA. Significant differences were found between pregnant and nonpregnant recipients at day 1 and 9 after transfer.
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