The current study was carried out to evaluate the cardio protective activity of Pulicaria Wightiana against Isoproterenol (ISO) induced myocardial infarction (MI). Pretreatment to the different groups were given for 30 days and ISO was administered at last two days with an interval of 24 hrs. Due to chronic ionotropy ISO induces MI. Blood was collected at the last day of experimental period and biomarkers were observed. The results indicate that the extract exhibited significant cardioprotective activity.