From the methanol extract of the Cardamine diphylla rhizome, methylethyl-(1), 2-methylbutyl-(2), 3-methylpentyl-(4), 3-indolylmethyl-(5), 1-methoxy-3-indolylmethyl-(6), 4-methoxy-3-indolylmethyl-(7) glucosinolates, and desulfo-2-methylbutylglucosinolate (3) were isolated. The structure elucidation of the compounds was performed by spectroscopic methods. The toxicity on brine shrimp larvae of the methanol extract of the C. diphylla rhizome was evaluated. In addition, the free-radical-scavenging activity of the crude extract was carried out by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.

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