The effects of BUdR on morphogenesis in Acetabularia mediterranea and on embryonic development (sea urchin, tunicate and amphibian embryos) have been studied.
In the presence of BUdR, cap development is affected in both the anucleate and nucleate fragment of the unicellular alga Acetabularia mediterranea. In the nucleate fragment, the initiation of cap formation can be inhibited. BUdR exerts various inhibitory effects on embryonic development. Modifications of sensitivity to BUdR during development are also observed: in Paracentrotus, a change in sensitivity towards the drug occurs at the 16 cell stage, while in amphibian eggs, a modification can be shown after the gastrula stage.
The significance of these observations is discussed.
Reçu le 17 septembre 1959 LA (lillÏTenciation des oeufs peut être suivie par l'évolution des particularités biochimiques que les embryons manifestent au cours de leur développement. L'em])loi de substances marquées par un isotope a déjà souvent révélé un parallélisme entre les dilTérenciations histologiques et biochimiques. Dans le cas des Amj)hibiens, ces éludes ont été limitées par l'existence d'un «coat » recouvrant les embryons et les rendant imperméables à un granti nombre de substances. Tel est le cas de l'uridine marquée au tritium, dont l'incorpora tion fait l'objet de ce travail. PROCÉDÉ EXPÉRIMENTAL ' Boursière de l'Institut Interuniversita ire des Sciences Nucléa ires.
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