In this study, we aim to determine the function of miR-124 on gastric cancer (GC) cells and the underlying mechanism that involves jaddeg1 (JAG1) and the Notch signaling pathway. GC tissues and adjacent tissues from 100 patients suffering from GC were selected. GC SGC-7901 and AGS cells were selected and grouped into control, mimic-NC, miR-124 mimic, inhibitor-NC, miR-124 inhibitor, and miR-124 inhibitor + si-JAG1 groups. RT-qPCR and a Western blotting assay were conducted to detect the expression of miR-124, JAG1, and Notch signaling pathway-related proteins (NICD, HES1, and HES5). MTS, wound-healing, transwell assay and flow cytometry were performed to detect cell proliferation, migration, invasion, cell cycle distribution, and apoptosis, respectively. Compared with adjacent tissues, a lower miR-124 expression and higher JAG1 expression were found in GC tissues. JAG1 is a direct target gene of miR-124. Compared with the control group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration, and proliferation in the miR-124 mimic group were decreased, while the apoptosis rate was increased and cells were arrested at the G0/G1 phase. Compared with the miR-124 inhibitor group, the expression of JAG1, NICD, HES1, and HES5, cell invasion, migration, and proliferation in the miR-124 inhibitor + si-JAG1 group were decreased, while the apoptosis rate and cell ratio at the G0/G1 phase were increased. The demonstration that miR-124 inhibits GC cell growth supports the concept that miR-124 functions as a tumor suppressor by a mechanism that involves translational repression of the JAG1 and the inhibition of Notch signaling pathway.

Background: Increasing evidence shows that gastrulation brain homeobox 2 (GBX2) is involved in multiple cancers. However, whether GBX2 has an effect on the lung adenocarcinoma remains unclear. In the present study, we investigated the functions of GBX2 on lung adenocarcinoma and explored the underlying mechanism.Methods: Public data were obtained from the TCGA (https://cancergenome.nih. gov) and Oncomine (http://www.oncomine.org) databases. GBX2 expression and its prognostic value were analyzed by bioinformatics methods. Relative mRNA and protein expression levels of GBX2 in lung adenocarcinoma cell lines were evaluated via a quantitative reverse transcriptase polymerase chain reaction and western blotting.Lung adenocarcinoma cell lines LTEP-a-2 and A549, respectively, were selected for gain and loss function of GBX2 assays. Cell viability was detected by CCK8 and clone formation experiments. Cell invasion and migration were assessed by Transwell assays. The effect of GBX2 on the AKT/extracellular signal regulated kinase (ERK) pathway was tested by western blotting.Results: Compared to adjacent tissues, GBX2 expression was up-regulated in lung adenocarcinoma tissues. High expression of GBX2 led to a poor survival and could be seen as an independent predictor for lung adenocarcinoma patients. Furthermore, down-regulation of GBX2 notably restrained the viability, invasion and migration abilities of A549 cells, whereas up-regulation of GBX2 in LTEP-a-2 cells presented the opposite outcomes. Furthermore, western blot indicated that down-regulation of GBX2 decreases the protein levels of phosphorylated (p)-AKT and p-ERK in A549 cells, whereas up-regulation of GBX2 shows the opposite effects in LTEP-a-2 cells. Conclusions:The results of present study indicate that GBX2 acts a cancerpromoting role to accelerate cell proliferation, invasion and migration partly by modulation of the AKT/ERK pathway in lung adenocarcinoma.

Through the review of relevant literature, theory and practice, consultation with the teacher. To explore the mechanism of invigorating spleen, invigorating lung and tongfu in the treatment of non-small cell lung cancer after postoperative radiotherapy and chemotherapy. The method of invigorating spleen, invigorating lung and tongfu organs is actually a dialectical combination of the theory of “cultivating earth and producing gold” and the theory of “using the six organs as a whole”, which has considerable curative effect. The method of invigorating spleen, invigorating lung and tongfu can improve the overall internal environment of the body, improve the quality of life and prolong the survival period for patients with non-small cell lung cancer after postoperative radiotherapy and chemotherapy.

Radiotherapy is a common treatment for abdominal and pelvic malignancies with bone metastases. However, some patients develop radiation enteritis (RE) during the treatment or 2 months or more after the treatment, which seriously affects their quality of life. At present, the curative effect of western medicine is poor. Professor Renting Li believes that radiation kills tumor cells but at the same time, it damages the healthy Qi of the human body. Radiation causes heat and poison to accumulate in the intestinal tract, consumes and hurts Yin fluid, burns the vein, leads to deficiency of healthy Qi and poor detoxification, as well as cause blood stasis after a period of time. All these result in the combination of heat, blood stasis, and poison, manifesting as abdominal pain, diarrhea, mucus in stools, purulent and bloody stools, etc. Therefore, Professor Renting Li proposed the use of traditional Chinese medicine retention enema combined with oral traditional Chinese medicine to reduce symptoms, remove toxins, and improve the quality of life of patients.

Exercise enhances the vascular function mainly by improving endothelial function. The way of exercise influences on hypertension‐induced vascular dysfunction remains unclear. Thus, the aim of this study was to examine the effects of acute severe exercise on insulin‐ and insulin‐like growth factor‐1 (IGF‐1)‐mediated vasorelaxation in spontaneously hypertensive rats (SHR). Three groups of rats were used in this study: SHR with exercise, SHR, and Wistar‐Kyoto rats (WKY) as control. The SHR with acute severe exercise group ran on treadmill at the speed of 28‐30 m/min until exhausted. Thoracic aortas were isolated to analyze vascular responses in organ baths. Selective inhibitors were used to examine the roles of endothelial nitric oxide synthase (eNOS) and phosphatidylinositol‐3 kinase (PI3K) in the vasorelaxation. Furthermore, the vascular response induced by sodium nitroprusside (SNP, a NO donor) was examined. Our results showed that, 1) insulin‐ and IGF‐1‐mediated vasorelaxation was significantly lower in SHR than WKY; 2) acute exercise ameliorated these adverse effects in SHR; 3) after treating either wortmannin (a PI3K inhibitor) or L‐NAME (a eNOS inhibitor), the differences of vascular responses among three groups were eliminated; 4) no significant differences of the SNP‐induced vasorelaxation were found among three groups. Our findings suggested that acute severe exercise could reverse the impairments of vascular function in hypertension.