A comparative study of phenolic acid extract from Quercus infectoria Gall using supercritical fluid extraction of CO2 (SCFE-CO2) with co-solvent methanol can produce yields, phenolic acid compositions, and differences in the extract's toxicity. SCFE-CO2 was carried out with the same weight of material (Q. infectoria gall) and run at a temperature of 60 oC and a pressure of 20 MPa with methanol as the co-solvent and an extraction time of 60 minutes. The extraction process extracts were identified by yield, phenolic acid composition using LC-MS/MS, total phenolic acid, and toxicity. The results of the SCFE-CO2 extraction technique with co-solvent methanol do not produce extracts that are toxic but have high yields and tannic acid compound (TPC). The SCFE-CO2 technique has small extract variability (div. sd. 0.1%), a high yield, a high tannic acid compound, a high TPC, and non-toxic, so SCFE produces extracts that have maintainable quality. The small amount of addition of methanol co-solvent can increase the quality of the extract using the SCFE-CO2 technique, and increasing the addition of methanol cannot increase yield.

A comparative study of phenolic acid extract from Quercus infectoria Gall using supercritical uid extraction of CO 2 (SCFE-CO 2 ) with co-solvent methanol can produce yields, phenolic acid compositions, and differences in the extract's toxicity. SCFE-CO 2 was carried out with the same weight of material (Q. infectoria gall) and run at a temperature of 60 o C and a pressure of 20 MPa with methanol as the cosolvent and an extraction time of 60 minutes. The extraction process extracts were identi ed by yield, phenolic acid composition using LC-MS/MS, total phenolic acid, and toxicity. The results of the SCFE-CO 2 extraction technique with co-solvent methanol do not produce extracts that are toxic but have high yields and tannic acid compound (TPC). The SCFE-CO 2 technique has small extract variability (div. sd. 0.1%), a high yield, a high tannic acid compound, a high TPC, and non-toxic, so SCFE produces extracts that have maintainable quality. The small amount of addition of methanol co-solvent can increase the quality of the extract using the SCFE-CO 2 technique, and increasing the addition of methanol cannot increase yield.