Background Due to the multitude industrial applications of ligninolytic enzymes, their demands are increasing. Partial purification and intensive characterization of contemporary highly acidic laccase enzyme produced by an Egyptian local isolate designated Alcaligenes faecalis NYSO were studied in the present investigation. Results Alcaligenes faecalis NYSO laccase has been partially purified and intensively biochemically characterized. It was noticed that 40–60% ammonium sulfate saturation showed maximum activity. A protein band with an apparent molecular mass of ~ 50 kDa related to NYSO laccase was identified through SDS-PAGE and zymography. The partially purified enzyme exhibited maximum activity at 55 °C and pH suboptimal (2.5–5.0). Remarkable activation for enzyme activity was recognized after 10-min exposure to temperatures (T) 50, 60, and 70 °C; time elongation caused inactivation, where ~ 50% of activity was lost after a 7-h exposure to 60 °C. Some metal ions Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Cd2+, Cr2+, and Mg2+ caused strong stimulation for enzyme activity, but Fe2+ and Hg2+ reduced the activity. One millimolar of chelating agents [ethylenediamine tetraacetic acid (EDTA), sodium citrate, and sodium oxalate] caused strong activation for enzyme activity. Sodium dodecyl sulfate (SDS), cysteine-HCl, dithiothreitol (DTT), β-mercaptoethanol, thioglycolic acid, and sodium azide caused strong inhibition for NYSO laccase activity even at low concentration. One millimolar of urea, imidazole, kojic acid, phenylmethylsulfonyl fluoride (PMSF), H2O2, and Triton X-100 caused activation. The partially purified NYSO laccase had decolorization activity towards different dyes such as congo red, crystal violet, methylene blue, fast green, basic fuchsin, bromophenol blue, malachite green, bromocresol purple eriochrome black T, and Coomassie Brilliant Blue R-250 with various degree of degradation. Also, it had a vast range of substrate specificity including lignin, but with high affinity towards p-anisidine. Conclusion The promising properties of the newly studied laccase enzyme from Alcaligenes faecalis NYSO strain would support several industries such as textile, food, and paper and open the possibility for commercial use in water treatment. It will also open the door to new applications due to its ligninolytic properties in the near future.

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Membrane biofouling in water purification plants is a serious issue of worldwide concern. Various chemical, physical, and biochemical processes are practised for membrane clean-up. A high-dosage treatment adversely affects the life expectancy of the membrane, and minimum dosage seems unable to deteriorate the biofilms on the membrane. It is reported that quorum quenchers like nitric oxide (NO) disrupt biofilm signals through metabolic rewiring, and also NO is known to be secreted by probiotics (good bacteria). In the present review, it is hypothesized that if probiotic biofilms secreting NO are used, other microbes that aggregate on the filtration membrane could be mitigated. The concept of probiotic administration on filtration membrane seeks to be encouraged because probiotic bacteria will not be hazardous, even if released during filtration. The fundamental motive to present probiotics as a resource for sequestering NO may serve as multifunctional bioweapons for membrane remediation, which will virtually guarantee their long-term sustainability and green approach.

Dietary and lifestyle planning decisions, dysbiosis in the composition of the microbial community and the production of bioactive metabolites by the gastrointestinal microbiota are all factors that have a significant influence on the mucosal integrity of the intestines and the progression of diseases like colon cancer.

No policy for the novel coronavirus 50% of COVID-19 dead have infected with a secondary bacterial infection. The threat of antimicrobial resistance could become a massive source of extra virus and death in our system of healthcare, as coronavirus pneumonia percentages grow far beyond throughput. Exploiting the antimicrobial-resistant gen in sewage for data collection and Analyzing human discharge waste passes via the drainage system to a treatment plant — is one approach scientists can follow for contagious diseases emitted in urine or human feces such as superbug serious long-lasting lung disease “COVID -19. Infections with polymicrobiomics biofilm have emerged as a major epidemiological issue for the reason that biofilm- are highly resistant to both multidrug resistance and immune system. Biofilm -substrate-related polymicrobes-infected artificial breathing machine and feeding tubes; act as a source of respiratory infection and tolerance for multidrug. More such reinforcement and implementation of probiotics are designed to improve the internal structure of gut microbiome and raising concerns of respiratory infections caused by microbial translocation and endocytosis. COVID-19 Patients Prerequisite to be investigated for pathogenic and opportunities infection microbiotia, not so much the COVID-19 .This home message is especially significant for those involved with risky behavior and hospital equipment, in infected people with weak immunity. The scenario whereupon gets more complicated