The present study examined the effect of filial therapy (FT) on interaction of deaf mothers with their hearing children. Participants were recruited from association and center of deaf people in Isfahan, Iran. The participants were 30 deaf mothers and their hearing children who were selected by convenient sampling method. They were randomly divided into the intervention and control groups and each group included 15 mothers and children. The intervention group received 11 FT weekly sessions for 3 months, while the control group did not receive the intervention. Mothers completed the Child-Parent Relationship Scale (CPRS) two times, at pre-and posttest phases. Results of multivariate analysis of covariance (MANCOVA) revealed that FT significantly influenced the interaction of mother-child scores, F(1, 29) = 42.69, p < .001. Accordingly, it can be suggested that interaction of deaf mothers with their hearing children was significantly increased following the FT intervention.