Dünyada sepsis tedavisi için doğal kaynaklı ilaçlar önerilmektedir. Bu nedenle, bu çalışmada, döteryumu azaltılmış suyun (DDW) tek başına ve Rosa damascena (RD) Mill. uçucu yağı ile birlikte etkisi oksidatif stres-antioksidan parametrelerin ve sepsisten kaynaklanan karaciğer hasarında siklooksijenaz-2 (COX-2) enflamatuvar genin ekspresyonunun değerlendirilmesi yoluyla belirlenmiştir. Gereç ve Yöntemler: Sıçanlar rastgele 5 gruba ayrılmıştır: 1) laparotomi grubu; 2) çekal ligasyon ve punksiyon (CLP) grubu; 3) DDW'ler (15 ppm ve 30 ppm dozlar) grupları; 4) DDW'ler (15 ppm ve 30 ppm dozlar) ile RD (100 mg/kg); 5) pozitif bir kontrol olarak indometasin (2 mg/kg). Tedaviler günlük olarak iki hafta boyunca yapılmış ve 15. günde CLP modeli oluşturulmuştur. Daha sonra hayvanlar öldürülmüş ve karaciğer dokuları histopatolojik ve biyokimyasal değerlendirmeler için ayrılmıştır.Objectives: Natural medicine has been proposed for treating sepsis worldwide. Therefore, in this study, the effect of deuterium-depleted water (DDW) alone and adjuvant with Rosa damascena Mill. (RD) essential oils was considered through the evaluation of oxidative stress-antioxidant parameters and the expression of cyclooxygenase-2 (COX-2) inflammatory gene in liver damage caused by sepsis. Materials and Methods: The rats were randomly divided into 5 groups: 1) laparotomy group; 2) cecal ligation and puncture (CLP) group; 3) DDW (15 ppm and 30 ppm doses) group; 4) DDW (15 ppm and 30 ppm doses) plus RD essential oil (100 mg/kg.bw); 5) indomethacin (2 mg/kg.bw) as a positive control. The treatments were daily administrated for 2 weeks and the CLP model was created on the day 15. Then, the animals were killed and their liver tissue was separated for histopathologic and biochemical assessment. Results: Our results demonstrated that the treatment of animals with DDW and DDW plus RD essential oil was effective due to the regulation of the oxidative stress-antioxidant parameters including lipid peroxidation, glutathione (GSH), GSH s-transferases, myeloperoxidase, ferric reducing ability of plasma and inflammatory parameters such as prostaglandin E2 and COX-2. Pathological studies also showed that sepsis led to the liver tissue injuries, which can be reduced by treatments. Conclusion: Sepsis caused oxidative stress in the liver tissue, but the administration of DDW and DDW plus RD essential oil can be useful to prevent and heal these injuries.

Objectives: Natural medicine has been proposed in treating of sepsis in the worldwide. So, in this study, the effect of deuterium-depleted water (DDW) alone and adjuvant with Rosa damascene Mill. essential oils (RD) was considered through the evaluation of oxidative stress/antioxidant parameters and the expression of cyclooxygenase-2 (COX-2) inflammatory gene in liver damage caused by sepsis. Materials and Methods: The rats were randomly divided into 5 groups: 1) laparotomy (LAP) group; 2) cecal ligation and puncture (CLP) group; 3) DDWs (15ppm and 30 ppm doses) groups; 4) DDWs (15ppm and 30 ppm doses) plus RD (100mg/kg.bw); 5) indomethacin (2 mg/kg.bw) as a positive control. The treatments were daily done for two weeks and creation of CLP model was occurred on the 15 th day. Then, the animals were killed and their liver tissue were separated for histopathologic and biochemical assessments. Results: Our results demonstrated that the treatment of animals with DDWs and DDWs plus RD were effective due to regulating the oxidative stress/antioxidant parameters including lipid peroxidation (LP), glutathione (GSH), glutathione s-transferases (GST), myeloperoxidase (MPO), ferric reducing ability of plasma (FRAP) and inflammatory parameters such as prostaglandin E2 (PGE2) and COX-2. Pathological studies also showed that sepsis led to the liver tissue injuries, which can be reduced by treatments. u n c o r r e c t e d p r o o f 2 Conclusion: Sepsis caused oxidative stress resulting in the liver tissue, but the administration of DDWs and DDWs plus RD can be useful to prevent and improve these injuries.