Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H2O2 (40 μM) and combination groups which incubated with constant dose of H2O2 (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.

Preliminary results of intraoperative photodynamic therapy with 5-aminolevulinic acid in dogs with prostate carcinoma. The Veterinary Journal. 2008; 178:202-207 As such the validity of the content of this article cannot be verified.The online version of the original article can be found at http://dx

The aim of this study was to evaluate the prevalence of acute and chronic inflammation, benign prostatic hyperplasia (BPH), and cancer of the prostate glands in the canine as a human model in prostate disorders. The study was carried out on 12 cases of different male dogs of terrier (50%), German shepherd (25%) breeds, and Greden (25%), and the age of the dogs ranged from 6 to 13 years (average age 7.8 ± 3.6). The bodyweight ranged from 3.6 to 7.9 kg. Signalment, clinical signs, and diagnostic tools such as ultrasonography, urinary cytology, and histopathology are presented. Dysuria was the most common clinical sign in this study and occurred in 10/12 canine (83.3%) included. Other clinical signs included lameness (5/12 canine, 41.6%) and constipation (3/12 canine, 25%). The range of duration of clinical signs was 5 days to 7 months. Moreover, in the present study, the urinary biochemical markers of different prostate lesions include blood, protein, and glucose and were detected in 11/12 cases (91.6%), 5/12 cases (41.6%), and 2/12 cases (16.6%), respectively. Taken together, sonographic data were classified into four groups based on histological diagnosis. In 7/12 cases (58.4%), the prostate appeared to have BPH lesions, and the remaining lesions included inflammation (3/12 cases, 25%), abscess (1 case, 8.3%), and adenocarcinoma (1 case, 8/3%) on ultrasound. In all cases, prostate tissue had an irregular echotexture. None of the dogs had sonographic evidence of sublumbar lymph node enlargement. Histopathologically, we looked at the prevalence of inflammation (33.3% chronic and 8.3% acute) and BPH (58.4%) in dogs of different ages and breeds, and also, we observed chronic inflammation in >20% of dogs, which was about 25% in 3 cases of the 12 cases referred. More chronic inflammation was associated with more BPH. The majority of the asymptomatic inflammation that is detected in the prostate is classified as chronic inflammation (i.e., as evidenced by the presence of monocytic and/or lymphoplasmacytic inflammatory cell infiltrates); however, acute inflammation is also observed to a lesser degree. Acute inflammation, as is typically evidenced by the infiltration of neutrophils, is classically an indicator of an infectious process. Finally, the patients included seven castrated, four castrated together with antibiotic therapy, and one castrated together with chemotherapy intact male dogs, which were treated with the mentioned cases. In conclusion, chronic prostatic inflammation could be a central mechanism in BPH progression, but the pathological features of tissue inflammation were different between BPH and prostate cancer (PCa). Nevertheless, the histological examination of prostate biopsies remains the only way to diagnose prostatic disorders.

In this study, a newly-designed diamine (DA) containing pyridine and carbazole groups was synthesized and the relevant polyamides (PAs) and polyimides (PIs) were prepared by polymerization reaction between the DA monomer and terephthalic acid, adipic acid, and pyromellitic dianhydride. In addition, polymer nanocomposites were prepared through a combination of the synthesized polymers with modified magnetite iron oxide nanoparticles. The synthesized compounds were fully characterized by hydrogen nuclear magnetic resonance (1HNMR), fourier-transform infrared (FTIR), field emitting scanning electron microscopy (FESEM), energy dispersive X-Ray (EDX), Transmission electron microscopy (TEM), Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA) analyses. All of the synthesized properties of compounds such as thermal stability, viscosity, elimination capability of heavy metals ions adsorption, and antibacterial activity were investigated. Polyamides and PI showed good solubility in aprotic solvents and the viscosity was in the range of 0.72–0.89 dL/g. Also, the glass-transition temperature (Tg) of synthetic polymers was in the range of 214–273°C and 10% weight loss temperatures (T10%) for the synthesized compounds were in the temperature range of 280–492°C in N2, and the percentage of residual ash between 61 and 74%. The adsorption rate of Hg2+, Co2+, Pb2+, Cr2+, and Cd2+ metal ions by PA containing terephthalic acid (PAT) and its nanocomposite (NCPAT) showed that nanocomposite has a better performance compared to PAT. The highest and lowest removal efficiency was related to Hg2+ and Pb2+ ion, respectively. Finally, antibacterial test results against Gram-positive and Gram-negative bacteria showed favorable inhibitory effects of all the synthesized polymers.

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