| Bovine brucellosis causes heavy losses in dairy animals. The current study was conducted to observe the prevalence of brucellosis in district Hyderabad, Pakistan. A total of Four hundred and Two (n=402) milk samples were collected from 30 towns during winter and summer seasons of 2014-2015 from Kundhi buffaloes. In winter, milk specimens (n=124) were collected from 12 towns and in summer (n=278) from 18 towns arbitrarily. All samples were investigated for Brucella abortus (B. abortus) antibodies using by milk ring test (MRT). An overall 1.99 percent (8/402) positive prevalence of B. abortus antibodies was detected in milk of Kundhi buffalo. From 124 milk samples collected throughout winter only 3 (2.41%) were found positive while, among 278 milk samples taken throughout summer, 5 (1.79%) were declared as positive. It was concluded that prevalence of brucellosis in Kundhi buffaloes was low in Hyderabad, Pakistan.

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The study was conducted to figure out the impact of exogenous Diethylstilbestrol on semen quality of Khundi buffalo bulls. Six Kundhi buffaloes of aged 5-6 years were divided into two groups, three bulls were placed in group-1 treated as experimental group and three bulls were kept as an untreated external control. 20mg of Diethylstilbestrol was injected intramuscularly to each experimental bull, parameters of semen and serum estrogen level was determined through ELISA. The comparative average semen volume, concentration, pH and motility in control and experimental animals were 4.7 vs. 5.39 ml, 1.43 vs. 1.27 x10 9 , 6.76 vs. 6.66 and 73 vs. 71% respectively; the color of semen was creamy in control animals than experimental animals, except than the concentration of bull semen of control and experimental animals the all studied seminal parameters of bulls were statistically non-significant. The serum estrogen level was significantly declined after Diethylstilbestrol treatment. It was concluded that the administration of exogenous estrogens in buffalo bull has no or little impact in the improvement of semen quality and resulted in decline of serum estrogen level bulls. Further studies at molecular level like relative gene expression of estrogen receptors at mRNA level and immunohistochemistry are required to confirm the specific cellular localization estrogen receptor in the testes of buffalo bulls.

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