Paca (Cuniculus paca Linnaeus, 1766) is the second largest rodent found in Brazil. The quality of the meat and a long tradition of hunting have contributed to the decline of the natural populations of this species. Hunting of paca is strictly prohibited in Brazil, but in spite of this restriction, no forensic tools are available for the identification of the meat. We describe an efficient method, based on single nucleotide polymorphisms of the cytochrome b gene, that can be used to differentiate biological material derived from paca from those of domestic species commonly used as sources of meat. The identification of the presence of C. paca in the samples was 100% reliable.Keywords: forensic science, Cytochrome b, wild rodent, molecular markers. Perfis diagnósticos do gene Citocromo b para a identificação molecular de paca (Cuniculus paca): implicações para a detecção e monitoramento de caça e comércio ilegalResumo Paca (Cuniculus paca Linnaeus, 1766) é o segundo maior roedor brasileiro. A qualidade da carne e a forte tradição da caça de subsistência são fatores que contribuem significativamente para o declínio das populações. Apesar da proibição a caça no Brasil, no momento ainda não há ferramentas disponíveis para identificar a carne e seus produtos como prova forense. Neste trabalho propomos um método eficaz de identificação, baseado em polimorfismos de único nucleotídeo no gene Citocromo b, objetivando diferenciar material biológico de paca das espécies domésticas comumente utilizadas como alimento no Brasil. A identificação das amostras de paca foram possíveis em 100% das amostras analisadas.Palavras-chave: ciência forense, Citocromo b, roedor selvagem, marcador molecular.
The objective of the present study was to evaluate the behavior of lactating Saanen dairy goats housed with or without the use of environmental enrichment technique. Twelve animals were split into two equal groups and assign to one of two treatments: stall with or without enrichment. Five objects were used to enrich the stalls: plastic PET bottles suspended and freely on the floor (both filled with corn as a visual and auditory stimuli), general cleaning brushes fixed to the wall of the stall, suspended tire, and a tree trunk for climbing. The behavior of the goats was observed during two hours for 10 consecutive days. The behaviors were recorded using focal-animal sampling method, observing each animal during 10minute period. Descriptive statistics were calculated plotting the results in behavior frequency charts. Animals on both treatments showed similar behaviors frequencies for food intake and self-cleaning. However, a higher frequency of stereotyped behaviors was registered in the treatment without environmental enrichment. The highest frequency of interaction was observed for suspended tire, indicating animal's preference for this object. In conclusion, animals on enriched environment show more different behaviors than animals in non-enriched environment and showed greater preference for some object over others.
Ciências da saúde: aprendizados, ensino e pesquisa no cenário contemporâneo está licenciado sob CC BY 4.0.Esta licença exige que as reutilizações deem crédito ao criador. Ele permite que os reutilizadores distribuam, remixem, adaptem e construam o material em qualquer meio ou formato, mesmo para fins comerciais. O conteúdo da obra e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores, não representando a posição oficial da Editora Amplla. É permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores. Todos os direitos para esta edição foram cedidos à Editora Amplla.
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