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A multlreflective absorption cell for CZE is fabricated and examined by both static and dynamlc measurements. The new cell Is characterlred by Improved sensittvlty as compared to conventlonal single-pass cells, wlth no increase In cell volume. A 40-fdd Improvement in sensltlvtty is obtalned when compared to a single-pass cell, wlth similar noise levels. A concentratton detectton ilmlt of 6.5 X lod M for Millant green

A method for the determination of the (6R)- and (6S)-stereoisomers of leucovorin using electrokinetic chromatography (EKC) in the affinity mode has been developed. Bovine serum albumin (BSA) is used as a run buffer additive to incorporate enantiomeric selectivity into the system. Protein-wall interactions are minimized by using a poly(ethylene glycol) (PEG) coated capillary. Chiral resolution is obtained in 12.5 min with efficiencies greater than 200,000 theoretical plates using BSA as an additive, while no resolution is obtained in the absence of BSA. A general equation is derived to calculate the free energy of interaction between the leucovorin isomers and the BSA molecule. This method represents a new means of obtaining thermodynamic data for substrate binding interactions and for the general study of drug cross-reactions and interactions of drugs with serum and other proteins.

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