Phosphoribosylpyrophosphate synthetase (PRS; EC 2.7.6.1) from Hevea brasiliensis Müll. Arg. latex was located in the cytosol. After purification, its apparent molecular weight under nondenaturing conditions was estimated at 200,000 f 10,000; a single band at 57,000 f 3,000 was detected after sodium dodecyl sulfatepolyacrylamide gel electrophoresis. l h e enzyme seemed to be a homotetramer. Its
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