The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth rinse, gingival crevicular fluid (GCF), and peri-implantitis fluid (PISF)) is demanding. Several hosts and microbial factors may influence their expression, release, and levels. The type of saliva/oral fluids utilized for the diagnostics affects the analysis. High sensitivity and specificities together with sophisticated methods and techniques are essential for valuable outcome. We describe here recently developed practical, convenient, inexpensive, noninvasive, and quantitative mouth rinse and PISF/GCF/chair-side/point-of-care (PoC) lateral-flow aMMP-8 immunoassays (PerioSafe and ImplantSafe/ORALyser) to detect, predict, and monitor successfully the course, treatment, and prevention of periodontitis and peri-implantitis, respectively. The tests have been independently and successfully validated to differentiate periodontal and peri-implant health and disease in Finland, Germany, Netherland, Sweden, Turkey, Nigeria, Malawi, and USA. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires additional studies utilizing these noninvasive screening, diagnostic, and preventive aMMP-8 PoC/chair-side technologies.

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Mg28 of a high specific activity was used in these studies. It was rapidly injected intravenously into 12 dogs and the concentration-time course curves in plasma were obtained. Urinary and fecal excretion was followed in seven animals. The dogs were killed 7 min to 68 hr following injection. Seventy-four tissues were sampled and assayed for Mg28 concentration. Plasma Mg24 was determined by flame spectrophotometry. With these data it was possible to follow the concentration-time course of Mg28 in the various tissues as well as to calculate their "exchanging" Mg24 content and effective rates of exchange. A variation in the pattern of uptake and in concentration of exchanging Mg24 was noted among the different tissues. During the interval from 24–68 hr, body exchanging Mg24 space and mass were found to range from 3.2–3.8 liters/kg and 4.9–5.7 mEq/kg, respectively. The biologic half-life of Mg28 in the dog estimated from excretion data was 11 days.