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Proximal tubules from the kidney of Thamnophis (garter snake) were perfused in vitro and unidirectional fluxes of lactate measured using L(+)-[U-14C]lactate, (lactate concentration, 1 mM). The lumen-to-bath (absorptive) flux (Jlb lact) significantly exceeded the bath-to-lumen flux (backflux) (Jbl lact) in each of 12 tubules (seven distal proximal and five proximal proximal). The flux ratio (Jlb lact/Jbl lact) was approximately 3.00. At flow rates of 13-16 nl/min and lactate concentration of 1 mM the net flux was about 1.60 pmol . min-1 . mm-1 in both proximal proximal and distal proximal segments. Both fluxes were decreased by perfusion at 5 degrees C. To determin e the contribution of metabolism of lactate to its absorption, Jlb lact was measured at 25 degrees C in 10 distal proximal tubules during perfusion with [14C]lactate, lactate concentration, 1 mM, and with [methoxy-3H]inulin. In these experiments, the amount of 14C found in the bath was 93% of the amount of 14C absorbed from the lumen. Chromatography showed that all of the 14C found in the bath was [14C]lactate. These data establish that in Thamnophis proximal tubule lactate absorption occurs against an electro chemical gradient by transport of the intact lactate molecule without significant metabolism.

For safe flight in the National Airspace System (NAS), either under the current interim rules or under anticipated longer-term regulatory guidelines facilitating unmanned aircraft system (UAS) access to the NAS, the UAS must incorporate technologies and flight procedures to ensure that neither people nor property in the air, on the ground, or on or in the water are endangered by the failure of an onboard component, by inappropriate unmanned aircraft (UA) response to pilot commands, or by inadvertent entry by the UA into prohibited airspace. The aircraft must be equipped with emergency recovery (ER) procedures and technologies that ensure that in the event of such a failure that the UA is recovered intact with minimal risk to other aircraft, people, or property. Finally, should ER procedures prove ineffective and it is impossible to recover the UA, the pilot-in-command and/or the UAS may engage flight termination (FT) procedures-activities to ensure that the UA is safely destroyed (should the UA be so equipped) or immediately grounded. Together ER and FT are referred to as emergency recovery and flight termination (ERFT). This paper presents a technology survey of ERFT technologies and procedures as applied toward unmanned aircraft.

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