Peroxisome proliferator-activated receptor Q Q (PPARQ Q) plays a central role in adipocyte di¡erentiation and insulin sensitivity. Although PPARQ Q also appears to regulate diverse cellular processes in other cell types such as lymphocytes, the detailed mechanisms remain unclear. In this study, we established a lentivirus-mediated short hairpin RNA expression system and identi¢ed a potent short hairpin RNA which suppresses PPARQ Q expression, resulting in marked inhibition of preadipocyte-toadipocyte di¡erentiation in 3T3-L1 cells. Our PPARQ Q-knockdown method will serve to clarify the PPARQ Q pathway in various cell types in vivo and in vitro, and will facilitate the development of therapeutic applications for a variety of diseases.

No abstract