Objectives: The aim of the present study was to evaluate the effect of platelet rich fibrin (PRF) on the healing of intra-bony defects. This evaluation was accomplished Clinically and radiograp-hically on patients. Subjects and Methods: A total of twenty one intra-bony defects in 14 patients were randomly divided into three groups; group A, treated with minced PRF placed inside the defects and covered with a PRF membrane, group B, treated with minced PRF placed in the defects and covered with a collagen membrane and group C, treated with xenograft placed in the defects and covered with a collagen membrane (control group). Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL) and radiographic parameters including linear radiographic measurements were measured at day of surgery (baseline) then 6 months post-operatively in all groups. Results: Clinical and radiographic results showed statistically significant differences between baseline and post operatively in all the groups. However inter-group comparisons showed no statistically significant differences after 6 months. Conclusion: The use of PRF as a regenerative material in managing periodontal intrabony defects resulted in pocket depth reduction, clinical attachment gain and defect fill with comparable results to bone graft and collagen membrane.