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Mice selected on the basis of an acute inflammatory response (AIR) can provide information about the immunopathological mechanisms of glomerulonephritis. We studied the differences between mice selected for a maximal AIR (AIRmax that attract more polymorphonuclear cells to the site of injury) or a minimal AIR (AIRmin that attract more mononuclear cells) in an experimental model of IgA nephropathy in order to investigate the effect of genetic background on glomerular disease progression and the participation of the monocyte chemoattractant protein-1 (MCP-1) chemokine. IgA nephropathy was induced by intraperitoneal ovalbumin injection and bile duct ligation in AIRmax and AIRmin mice. Histological changes, urinary protein/creatinine ratio, serum IgA levels, immunofluorescence for IgA, IgG and complement C3 fraction, immunohistochemistry for macrophages and MCP-1, and MCP-1 levels in macerated kidney were determined. Mesangial IgA deposition was seen only in AIRmin mice, which presented more renal lesions. Increased serum IgA levels (1.5 ± 0.4 vs 0.3 ± 0.1 mg/mL, P < 0.001), high glomerular MCP-1 expression and decreased monocyte/macrophage infiltration in the interstitial area (0.3 ± 0.3 vs 1.1 ± 0.9 macrophages/field, P < 0.05) were detected in AIRmin mice compared to AIRmax mice. No glomerular monocyte/macrophage infiltration was detected in either strain. In spite of the absence of IgA deposition, AIRmax mice presented discrete or absent mesangial proliferation. The study showed that there are differences between mice selected for AIRmax and AIRmin with respect to serum IgA levels, histological damage and MCP-1 chemokine production after ovalbumin injection in combination with bile duct ligation.

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Purpose : Inferior turbinates chemosurgery with trichloroacetic acid TCA has been assessed histopathologically in allergic rhinitis by comparing bilateral inferior turbinates with both sides treated by turbinectomy and only the deviation free side treated with TCA due to a deviated nasal septum.Because such deviation may influence comparison results, we assessed its influence on Th2 and Th1 cell infiltration of specimens. Subjects : Specimens were bilateral inferior turbinate membranes from 13 subjects with non treated HD allergy. Methods : The two types of cells were counted immunohistochemically per 1mm 2 per specimen. Two way analysis of variance used the deviation and cell type as primary factors. Results : The cell type, but not the deviation factor, was significant at p 0.01. Conclusion : The deviation factor was not related to Th2 or Th1 cell infiltration, but has not been accounted for conventional assessment. Our results confirmed that necessity of the deviation factor need not be considered in assessing TCA treatment efficacy.