Genetic diversity can be improved using the gamma ray irradiation to obtain the resistant plants to the disease. The Changes occur can be analyzed at the beginning of plant growth using molecular analysis; RAPD technique (Random Amplified Polymorfism DNA). This study aims to determine the genetic diversity produced by gamma rays irradiated and given the poly ethilene glycol given in plantlets to increase chrysanthemum resistance to Medium plain. The study used four primers tested on four treatments that were not irradiated plantlets and induced the poly ethilene glycol, gamma rays irradiated 25 gray un induced poly ethilene glycol, irradiated and induced the poly ethilene glycol 15 and 30%.The results showed a decrease of control diversity of genetic 0.20 to 0.30 in 25 gray irradiation treatment with given the poly ethilene glycol 15%.The use of the RAPD technique to determine genetic diversity resulting from gamma ray irradiation on the level of plantlets. Gamma ray irradiation treatment causing mutations in all treatments tested. Plantlets of 25 gray irradiation induced with 15%poly ethylene glycol / l showed changes in DNA structure.

Water apple citra is a local fruit that contains a complete mineral. Production of water apple citra can be increased through vegetative propagation, one of them is cuttings. The growth of cuttings can be stimulated using plant growth regulators and growing medium. This study aims to determine the effect of plant growth regulators type and growing medium as well as the interaction between these factors. The research was conducted on March until May 2016 at the garden experiment, Faculty of Agriculture, UPN “Veteran” Yogyakarta. This research used Randomize Completely Block Design (RCBD) factorial pattern with three replications. There were two factors studied, the first factor was the type of plant growth regulators consist of root-up, rootone–F, atonic and without plant growth regulatos, the second factor was growing medium consist of the river sand, floral foam, and river sand+compost. The observed data were analyzed by analysis of variance (ANOVA) and continued by DMRT at 5% real level. The results showed that rootone-F could increase percentage of cutting life and root length. Growing medium of river sand+compost could increase percentage of cutting life. There were an interaction due to the combination of treatment on the observation variable such as root length and root volume.was analyzed by using Duncan Multiple Range Test (DMRT) at 5% level. The result showed that there were an interaction between liquid organic fertilizer various and Trichoderma sp. on the plant height at 42 days (the days after planting), the leaves number at 42 days (the days after planting), the root lenght, the roots number, and the crop weight of red cabbage. The treatment combination of cow urine and Trichoderma sp. gave the best crop weight compared to other treatments. There was no significant effect on vitamin C and vitamin A content in the red cabbage.Keyword: water apple citra, plant growth regulators, growing medium

The economic value of the Abaca banana is on the trunk which contains fiber for valuable textile and paper industry raw materials. The multipurpose fiber and its prospects are quite good, so the abaca plant gets a lot of attention from various community groups both private, state-owned, cooperative and farmers. Propagation through multiplication humps is very low, planting material is easily damaged in transportation, is not durable and requires large space so that transportation costs are high. One alternative to overcome the provision of healthy, large and fast seedlings is to use seedlings from tissue culture. The aim of the study is to get the right thiamin and leaf fertilizer concentrations at the acclimatization stage. The experimental design used was a 2-factor Complete Randomized Design, Thiamin concentration (1 ml / l, 2 ml / l, 3 ml / l) and leaf fertilizer (0.5 ml / l, 1 ml / l, 1.5 ml / l). The data obtained were analyzed for diversity at 5% level and further tests using Duncan Multiple Range Test (DMRT) level of 5%. The results showed no interaction between thiamin and leaf fertilizer, Thiamin 2 ml / l gave the best results on all parameters and 1.5 ml / l leaf fertilizer could increase plant height and the number of leaves of abaca banana plants.Keywords: Thiamin, leaf fertilizer, abaca banana, acclimatization

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

Koja is a widely known variety of bananas but the availability of high-quality seeds has not been able to meet the market demand. This problem is overcome by using tissue culture, meanwhile, the success of the culture is largely determined by the acclimatization stage or adaptation of plants from heterotrophs to autotrophs. Therefore, this study aims to determine the interaction between the concentration of foliar fertilizer and the length of containment as well as the appropriate concentration for the growth of Koja banana plantlets. This is a field experiment carried out with a 2-factors split-plot design. The main plot was the foliar fertilizer concentration namely M1 = 2 ml/L; M2 = 4 ml/L; M3 = 6 ml/L, while the subplots were the length of containment namely E1 = 10 days; E2 = 20 days; E3 = 30 days. Based on the results, there was an interaction between the concentration of foliar fertilizer 4 mL/L and the length of containment of 20 days on the height, root volume, as well as fresh and plant dry weight.