Kelelahan kerja perawat yang tidak dapat diatasi akan menimbulkan berbagai permasalahan kerja yang fatal. Penelitian ini bertujuan menilai perbandingan tingkat kelelahan perawat di ruang ICU RSD Idaman Banjarbaru dan RSUD Ratu Zalecha Martapura. Penelitian menggunakan rancangan penelitian komparatif. Populasi dalam penelitian ini perawat di ruang ICU RSD Idaman Banjarbaru sebanyak 15 orang dan perawat di ruang ICU Ratu Zalecha Martapura sebanyak 16 orang, yang diambil dengan teknik cluster sampling, data dianalisis dengan uji Mann-Whitney. Terdapat perbedaan antara Tingkat kelelahan perawat di ICU RSD Idaman Banjarbaru dengan mayoritas ringan sedangkan RSUD Ratu Zalecha Martapura dengan mayoritas sedang dengan P Value 0.015. Penting bagi pihak manajemen rumah sakit untuk mengkaji kesesuaian jumlah perawat dan beban kerja sebagai cara meningkatkan pelayanan diruangan. Kata-kata kunci : kelelahan perawat; kelelahan fisik; kelelahan psikologis ABSTRACT Fatigue of nurses that not resolved would cause various problems. This research aims to assess the comparative level of nurse fatigue in intensive care unit of idaman banjarbaru hospital and ratu zalecha martapura hospital. This research used comparative. The population of this research were nurses of ICU in Idaman Banjarbaru Hospital as many as 15 people and nurses of ICU Ratu Zalecha Martapura Hospital as many as 16 people, the data presented by cluster sampling; analyzed with the Mann-Whitney test. This study showed difference difference between level of fatigue of both hospital. The majority of nurses fatigue in ICU RSD Idaman Banjarbaru has light fatigue while the majority of nurses in ICU Ratu Zalecha Martapura had mild fatigue with P Value 0.015. For hospital management must to assess the suitability of the number of nurses and workloads to improve the service.