The aim of this study was to develop and validate a novel serological test employing surface plasmon resonance (SPR), that was designed for use on the Biacore Q apparatus to detect antibodies against Salmonella Spp in pig sera. Salmonella Spp antigens (sero-groups B and D) were immobilized onto CM-5 sensor chips (Biacore). Test conditions and apparatus adjustments were optimized. Subsequently, validation was done using positive and negative ("in house") reference sera as well as 990 field sera collected at slaughter. A set of 990 sera originating from finishing pigs were tested in the SPR assay and ELISA. Overall agreement between the two tests amounted to 92%. From these results it is concluded that the SPR assay in conjunction with the Biacore Q apparatus is suitable for screening slaughter pigs against Salmonella in a surveillance program.