Aspergillus is an opportunistic fungus that usually colonizes preexisting lung cavities, especially tuberculous ones. Colonization of a pulmonary sequestration by this germ is exceptional, with just 14 cases reported in the world literature, most of them in Asia. A case is presented of a 48-year-old woman with pleuritic thoracic pain. Simple chest radiology revealed a lower right pulmonary tumor with clear margins and a calcium-type density. CT showed it to correspond to a 6 x 5-cm hypodense mass, which was enhanced at the periphery with intravenous contrast. Aspiration puncture yielded a greenish-yellow pus and the microscopic study strongly suggested Aspergillus, confirmed by culture as Aspergillus fumigatus. Surgery revealed an infected pulmonary sequestration at the lower right lobe, and a lobectomy was performed.