To sustain the competitive edge, competent Human Force are required both at present and in the future and to achieve that, best practices are in reconciling the need of the employees in terms of health, and safety in workplaces in an organization. This study examines best practices, employee's occupational health and safety and the mediating role of organisational culture in a glass manufacturing company. The study uses responses from 350 employees of BETA Glass Company in Nigeria relying on regression analysis techniques and PLS structural equation modelling to test four hypotheses. This study validates the assumptions that training and development dimension of best practices positively impacts employee's health and safety in the workplace. This study affirms that leadership quality dimension of best practices fosters employee's health and safety in the workplace. Further, safe work procedures a dimension of best practices promote employee's health and safety in the workplace. Similarly, the result suggests that organisation culture mediates the relationship between best practices and employee's health and safety in the workplace. We, therefore, conclude that the relationship between best practices and employee's health and safety in the workplace is both linear and indirect..