This study proposes a liquid chromatography (RPHPLC) method for quantitative analysis of the most widely prescribed combination of Paracetamol, Propyphenazone and Caffeine in tablet dosage form in presence of Rasagiline as an internal standard. The chromatography was performed on a Gracesmart C18 column (5µm, 250mm×4.6mm i.d.) using the mixture of water and 2-propanol in the ratio 80:20 v/v as mobile phase. The pH of aqueous phase was adjusted to 3.0 with 1% o-phosphoric acid and a flow rate was maintained at 1.5ml/min. The analytes were detected by measuring the absorbance at 210nm. The chromatographic analysis time for each sample was kept about 20min as Rasagiline, Paracetamol, Caffeine and Propyphenazone were eluted at 1.63, 2.36, 3.17 and 11.22 min respectively. The calibration plots were obtained between 3-90µg/ml for Paracetamol, 1.5-45µg/ml for Propyphenazone and 0.5-15µg/ml for Caffeine. The method was validated in accordance with ICH norms and method was found to be specific, accurate, precise and easy to use for routine analysis.

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This study proposes a liquid chromatography (RP-HPLC) method for quantitative analysis of the most widely prescribed combinations of Ranitidine hydrochloride, Domperidone and Magaldrate in tablet dosage form in the presence of Metronidazole as an internal standard. The chromatography was done on a C18 column (250 mm × 4.6 mm, 5 (m) as stationary phase and Methanol: Potassium dihydrogen ophosphate (65:35 % v/v, pH 3.0 adjusted to 1% OPA) as mobile phase at a current rate of 1mL/min and measured at 227nm. Retention time was found to be 2.68min, 3.66min and 5.25min for RH, DM and MZ respectively. The assay of RH and DM in the formulation was found to be 100.33% w/w and 99.53% w/w respectively. The linearity range for RH was 3-150 µg/mL and for DM was 0.2-10 µg/mL. The developed method was validated in accordance to ICH guidelines.

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