Penentuan jenis kelamin pada beberapa spesies burung cukup sulit dilakukan dikarenakan jantan dan betina memiliki ciri morfologi yang sama (monomorfik), salah satunya famili Columbidae. Teknik penentuan jenis kelamin burung secara molekuler yang populer adalah metode Polymerase Chain Reaction (PCR) dengan gen target Chromo Helicase DNA-binding (CHD), namun keberhasilan amplifikasi gen target pada PCR tersebut dipengaruhi salah satunya kesesuaian DNA template dengan primer yang digunakan. Penelitian ini bertujuan untuk mengevaluasi 5 jenis primer PCR P2/P8, 2550F/2718R, CHD1F/CHD1R, 1237L/1272H dan CHD1LF/CHD1LR untuk menentukan jenis kelamin famili Columbidae. Penelitian ini dilakukan dengan menguji 5 jenis primer tersebut di atas pada sampel DNA tiap pasang jantan dan betina dari burung merpati, balam jambi, punai, derkuku, dan perkutut. Hasil penelitian diketahui primer CHD1LF/CHD1LR menunjukkan hasil terbaik dan direkomendasikan untuk menentukan jenis kelamin famili Columbidae.
<p><span class="fontstyle0">Objective: </span></p><p><span class="fontstyle2">Sex determination of birds is crucial role in breeding and conservation purposes. Genomic approaches by using basis of bird sex polymorphism are accepted to generate an accurate sexing procedure. The PCR method is commonly used and known to provide accurate test results in determining the sex of birds, but its successes is strongly influenced by the compatibility of the primers used with the DNA template. This study aimed to determine the potential use of CHD1LF/CHD1LR targeting CHD gene fragment in 4 species from Phasianidae, Psittacidae, Estrildidae, and Passeridae families.</span></p><p><span class="fontstyle2"><br /></span><span class="fontstyle0">Method: </span></p><p><span class="fontstyle2">Blood samples (n= 30) from representatives of species in 4 families above were collected for DNA isolation, then PCR test was performed using CHD1LF/CHD1LR primers. The PCR results obtained were compared with positive controls, necropsy examination and information from bird sellers.</span></p><p><span class="fontstyle2"><br /></span><span class="fontstyle0">Result: </span></p><p><span class="fontstyle2">The results showed that the primer CHD1LF/CHD1LR could be used in Phasianidae,<br />Psittacidae, Estrildidae, and Passeridae families. The PCR product was confirmed to be same as well as the positive control and necropsy results. It is demonstrated that male birds showed a single band (474 bp), while double bands (474 and 319 bp) were observed in female birds. Different accuracies were observed during this study between molecular approach and manual sexing by bird seller i.e:<br />sparrows (46%) and finches (50%), while the accuracy of parakeets was 75%, and quails 80%.</span></p><p><span class="fontstyle2"><br /></span><span class="fontstyle0">Conclusions: </span></p><p><span class="fontstyle2">Primer CHD1LF/CHD1LR can be used to determine the sex of birds from the families<br />Phasianidae, Psittacidae, Estrildidae, and Passeridae. Our study showed that molecular-based method is valuable and able to reduce error rate to 100% in bird sexing procedures.</span> <br /><br /></p><p> </p>
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